Monday, October 14, 2013

Bio Lab-Organic Molecules

In this experiment, we were trying to moderate which essential jots were present in three different transcendental protein dietary supplements, and if they were or were non in the first place protein based, or if they contained other molecules as well. Many dietary supplements just claim to be full of proteins, but atomic identification number 18 they re all toldy? In this experiment, we hope to turn back if this is true or not. In edict to determine the essential molecules present in from individually matchless of the different unappreciated dietary supplements, we used cardinal reagents: Lugols, Benedicts, Ninhydrin, and Biuret. We for distributively one chose matchless of the reagents and ran three different exertions for each reagent. In each trial we had five tribulation tubes: wiz with 5 mL of unknown 1, one with 5 mL of unknown 2, one with 5 mL of unknown 3, one with 5 mL of draw to show the prohibit reaction, and one with 5 mL of each reagents coop erative organic molecule (Lugols-starch, Ninhydrin-Glycine, Benedicts-Glucose, Biuret-Protein) to show the po amazeive reaction. Also for each test tube in each of the three trials, we added one mL (2 mL for Biuret) of reagent. For Ninhydrin and Benedicts reagent, all of the tubes in each trial must devolve on in a water bath: Ninhydrin for 5-10 minutes at 80 degrees celcius and Benedicts for 4-7 minutes at 65 degrees celcius.
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After mixing all the solvents and allowing Ninhydrin and Benedicts to razz in the water baths for the circumstantial amount of time, cover each tube of all reagents and their trials with a strip of parafilm, mix the solution well, and allow the solution to! sit while the color transpose occurs. After allowing each of the solutions of each trial to mix properly, determine which color each of the solutions are and compare them to the positive and negative of each reagent to see if each of the unknowns contain the specific organic molecules that are associated with that reagent. Controls Colors | Ninhydrin| Lugols| Biuret| Benedicts| Negative| Clear| Yellow/brown| Clear/ miniatureBlue| Light Blue|...If you want to get a full essay, order it on our website: BestEssayCheap.com

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