Slime Mold Lab Report

[author][professor][subject][date]AbstractThe Dictyostelium purpureum was shelld on antithetical media containing (1 ) Escherichia coli (2 ) dirty money (3 ) potato (4 ) banana (5 ) gelt and glucose and (6 )water . Fruiting body formation was observed or all in all colonies with die-off at water completely . There was observable migration or selective chemotaxis for diet preferences for E . coli and simpler sugarsI . IntroductionThe Dictyostelium purpureum ( Domain : Eukarya Phylum : Amoebozoa Class Mycetozoa , Dictysliida ) belongs to a group of dictyostelids commonly know as cellular begrime moulds . Their lifetime involves two stages ? the amoeba and smoke phase . At favorable environment conditions and pabulum supply they accede to their amoeban form otherwise at food scarcity , they center to form a slug . The m echanism for cell aggregation for the slime mold travel by ring reaction , whereby the amoeba , releases cAMP signals as stress retort from food scarcity Others would migrate to cAMP concentrations .The slug is one grown migratory aggregate demeanor an anterior and posterior , with chemotactic phototactic , thermotactic rejoinder . Given proper environmental conditions , the payoff body (a exploitation /development of slug thatcontains spores , willing become the new amoebaIn the performed science lab exercise on Dictyostelium purpureum is plated on different meadia bearing different food . The response of Dictyostelium sp . and the life motorcycle was observed on the different food for thought mediaII .MethodsUsing aseptic techniques , same amount of neat , young gloss of Dictyostelium purpureum was plated on different media containing the subsequent provender : Petri plate (1 ) Escherichia coli (2 ) popsicle (3 potato (4 ) banana (5 ) sugar and glucose and (6 )wa ter . The Dictyostelium was incubated benea! th room temperature conditions , 320C , for a duration of one week . For apiece day of the week , the Petri plates were observed for the formation of cell aggregates and fruit structures . Growth was overly measured using a with 1 cm broadcast on it .

Possible contamination is also noted . whole observations were put discomfit on Table 1III . Results and DiscussionThe life cycle of Dictyostelium purpureum and its response to different nutrient media were observed and recorded in Table1Table 1 shows that during at that place was no formation of fruit body on Day 1 and Day 2 . This indicates that the amoeba was still prevalent in the different media and that in that location was no scarcity of food yet . Upon culture plate of the organisms , the individual or the single-celled amoeba adapts to the environmental conditions of the irrelevant media , get rollings to grow and develop as there argon copiousness of food . Amoeba cannot be discerned by the in the buff core . It is only the fruiting body structure which would be overt . At Day 2 , there ar likely food depreciation to near scarcity because fruiting bodies may pay started to grow for the culture on bread and water . For the culture on bread and banana , the start of fruiting body formation may have been because the amoeboid on the medium has difficulty breaking down the entangled nutrient of the bread and which is consist mainly of obscure carbohydrate...If you inadequacy to get a full essay, order it on our website: BestEssayCheap.com

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